| Father | Sir John Osborne (1517 - 1597) |
| Mother | Lady Susanna Ann Friend (1524 - 1591) |
| Sibling | Sir John Osborn (1551 - 1628) |
| Sibling | Lord Edward Osborne (1538 - 1592) |
| Sibling | Thomas Osborne (1543 - 1616) |
| Sibling | John Osborne II (1543 - 1616) |
| Sibling | Jane Osborne (1545 - 1619) |
| Sibling | Mary Osborne (1549 - 1631) |
| Sibling | Elizabeth Osborne (1549 - ) |
| Sibling | Alice Osborne (1551 - ) |
| Sibling | Jonas Osborne (1553 - ) |
| Sibling | Agnes Osborne (1554 - 1600) |
| Sibling | Josiah Osborne (1554 - ) |
| Sibling | Peter Osborne (1556 - ) |
| Sibling | Chistopher Osborne (1557 - 1600) |
| Sibling | Nicholas Osborne (1558 - ) |
| Sibling | Henri Osborne (1559 - ) |
| Sibling | Richard Osborne (1561 - ) |
| Sibling | Anne Osborne (1563 - 1657) |
| Sibling | Osborne (1564 - 1657) |
| Sibling | Maudin Osborne (1570 - ) |
| Sibling | Catherine Osborn (1580 - ) |
| Sibling | Dorothy Osborne (1583 - ) |