| Spouse | Lady Susanna Ann Friend (1524 - 1591) |
| Child | Sir John Osborn (1551 - 1628) |
| Child | Lord Edward Osborne (1538 - 1592) |
| Child | Julian Osborne (1542 - 1642) |
| Child | Thomas Osborne (1543 - 1616) |
| Child | John Osborne II (1543 - 1616) |
| Child | Jane Osborne (1545 - 1619) |
| Child | Mary Osborne (1549 - 1631) |
| Child | Elizabeth Osborne (1549 - ) |
| Child | Alice Osborne (1551 - ) |
| Child | Jonas Osborne (1553 - ) |
| Child | Agnes Osborne (1554 - 1600) |
| Child | Josiah Osborne (1554 - ) |
| Child | Peter Osborne (1556 - ) |
| Child | Chistopher Osborne (1557 - 1600) |
| Child | Nicholas Osborne (1558 - ) |
| Child | Henri Osborne (1559 - ) |
| Child | Richard Osborne (1561 - ) |
| Child | Anne Osborne (1563 - 1657) |
| Child | Osborne (1564 - 1657) |
| Child | Maudin Osborne (1570 - ) |
| Child | Catherine Osborn (1580 - ) |
| Child | Dorothy Osborne (1583 - ) |