| Father | Richard I. Middleton (1509 - 1575) |
| Mother | Joanna J Dryhurst (1525 - 1565) |
| Sibling | Sir Hugh Myddelton 1st Baronet (1560 - 1631) |
| Sibling | Jane Middleton (1541 - ) |
| Sibling | Ales Middleton (1545 - ) |
| Sibling | William Middleton (1546 - 1621) |
| Sibling | Lucy Middleton (1546 - ) |
| Sibling | Sir Thomas Myddelton (1550 - 1631) |
| Sibling | Richard Myddelton (1553 - 1625) |
| Sibling | Charles Myddelton (1555 - 1621) |
| Sibling | Elias Myddelton (1557 - 1631) |
| Sibling | Margrett Myddelton (1563 - 1588) |
| Sibling | Robert Myddelton (1563 - 1616) |
| Sibling | Grace Myddelton (1564 - 1588) |
| Sibling | Fulke Myddelton (1564 - 1629) |
| Sibling | Pyrs Myddelton (1565 - 1594) |
| Sibling | Alice Myddelton (1565 - ) |
| Sibling | Peter Middleton (1600 - ) |
| Sibling | Edward Myddelton ( - 1620) |