| Spouse | Joanna J Dryhurst (1525 - 1565) |
| Child | Sir Hugh Myddelton 1st Baronet (1560 - 1631) |
| Child | Simon Myddelton (1541 - 1594) |
| Child | Jane Middleton (1541 - ) |
| Child | Ales Middleton (1545 - ) |
| Child | William Middleton (1546 - 1621) |
| Child | Lucy Middleton (1546 - ) |
| Child | Sir Thomas Myddelton (1550 - 1631) |
| Child | Richard Myddelton (1553 - 1625) |
| Child | Charles Myddelton (1555 - 1621) |
| Child | Elias Myddelton (1557 - 1631) |
| Child | Margrett Myddelton (1563 - 1588) |
| Child | Robert Myddelton (1563 - 1616) |
| Child | Grace Myddelton (1564 - 1588) |
| Child | Fulke Myddelton (1564 - 1629) |
| Child | Pyrs Myddelton (1565 - 1594) |
| Child | Alice Myddelton (1565 - ) |
| Child | Peter Middleton (1600 - ) |
| Child | Edward Myddelton ( - 1620) |