| Father | Major Thomas Hayward Mitchell of the Muirhead Separatist Movement (1566 - 1627) |
| Mother | Margaret "Maria" Cromwell Williams Vochin (1568 - 1625) |
| Sibling | Experience Mitchell of the Mayflower (1602 - ) |
| Sibling | Major Deacon James Matthew Mitchell (1591 - 1646) |
| Sibling | Grace Mitchell (1583 - 1622) |
| Sibling | Henry Mitchell (1600 - 1635) |
| Sibling | Matthew Mitchell (1590 - 1646) |
| Sibling | Sibil Mitchell (1593 - 1618) |
| Sibling | Susan Mitchell (1604 - 1650) |
| Sibling | Thomas Francis Mitchell (1599 - 1666) |
| Sibling | Elizabeth Mitchell (1590 - 1658) |