| Father | Sir William Cunningham Earl of Glencairn (1532 - 1579) |
| Mother | Lady Janet Laverne Huntly Gordon of Lochnivar (1528 - 1576) |
| Sibling | Sir James Cunningham Earl of Glencairn - Scottish Peer Privy Council (1550 - 1630) |
| Sibling | Lady Isabelle Cunningham (1554 - 1586) |
| Sibling | Arche Archibald Cunningham (1554 - 1580) |
| Sibling | Lady Margaret Cunningham (1554 - 1595) |
| Sibling | Lady Katherine Elizabeth Crawford Countess of Lochinver (1555 - 1611) |
| Sibling | Elisabeth Cunningham (1555 - 1613) |
| Sibling | Lady Marion Cunningham (1556 - 1661) |
| Sibling | Lady Jean Katherine Cunningham (1558 - 1604) |
| Sibling | Patrick Cunningham (1559 - 1644) |
| Sibling | Alexander Corsehill Cunningham (1560 - 1646) |
| Sibling | Susannah Napier Cunningham (1561 - ) |
| Sibling | Robert Cuningham (1561 - 1631) |