| Father | Emanuel KLINEFELTER (1832 - 1906) |
| Mother | Mary Elizabeth SCHAEFER/SHAFFER (1836 - 1882) |
| Sibling | John William O. KLINEFELTER (1855 - 1926) |
| Sibling | Samuel KLINEFELTER (1857 - 1936) |
| Sibling | Tilghman/Thielman KLINEFELTER (1858 - 1910) |
| Sibling | Marietta/Mary KLINEFELTER (1860 - ) |
| Sibling | Elizabeth Mahala KLINEFELTER (1862 - 1925) |
| Sibling | George KLINEFELTER (1863 - 1947) |
| Sibling | Dianna/Denia Adelia K. Idena KLINEFELTER (1866 - 1949) |
| Sibling | Lyman KLINEFELTER (1869 - 1915) |
| Sibling | Simon KLINEFELTER (1869 - 1943) |
| Sibling | Jesse Leroy KLINEFELTER (1871 - 1956) |
| Sibling | Alfred/Alvin Albert KLINEFELTER (1877 - 1964) |
| Sibling | John KLINEFELTER ( - ) |