| Father | Robert Johnston (1759 - 1820) |
| Mother | Elizabeth Awbrey (1760 - 1821) |
| Sibling | Posey Johnston (1796 - 1859) |
| Sibling | Benjamin Johnston (1782 - 1827) |
| Sibling | Robert Dugan Johnston (1783 - 1846) |
| Sibling | John Johnston (1785 - 1864) |
| Sibling | Areathusca Ann Johnston (1787 - ) |
| Sibling | Mary Johnston (1789 - 1808) |
| Sibling | William Johnston (1790 - 1791) |
| Sibling | Elizabeth Johnston (1792 - 1793) |
| Sibling | Samuel Awbrey Johnston (1794 - 1859) |
| Sibling | Margaret Johnston (1798 - 1858) |
| Sibling | Thomas Dougan Johnston (1800 - 1882) |
| Sibling | Chandler Awbrey Johnston (1804 - 1827) |