| Father | William Thomas Chambers Sr (1777 - 1871) |
| Mother | Catherine Lakey "Katie" Lawson (1792 - 1838) |
| Sibling | James Riley Chambers (1809 - 1888) |
| Sibling | Lamanda L. "Amanda" Chambers (1809 - 1865) |
| Sibling | Pleasant "Ples" Chambers Sr (1813 - 1887) |
| Sibling | John Riley Chambers (1815 - 1894) |
| Sibling | Sarah Elizabeth Chambers (1822 - 1904) |
| Sibling | William Thomas Chambers Jr (1825 - 1893) |
| Sibling | Daniel Chambers (1826 - 1853) |
| Sibling | William Alexander "Elick, Alex" Chambers (1829 - 1908) |
| Sibling | David C. Chambers (1830 - 1880) |
| Sibling | Jeremiah Jackson Chambers (1835 - 1857) |
| Sibling | 2LT Francis Marion Chambers (1840 - 1915) |