| Father | William Cecil Chambers Sr (1795 - 1876) |
| Mother | Judith Elizabeth C. Metcalfe (1794 - 1876) |
| Sibling | William Cecil Chambers Jr (1813 - 1870) |
| Sibling | Elizabeth Chambers (1815 - 1886) |
| Sibling | Ezekiel "Zeke" Chambers (1816 - 1865) |
| Sibling | Julius Amos Chambers Sr (1818 - 1885) |
| Sibling | Sarah Jane Chambers (1820 - 1862) |
| Sibling | Margaret Chambers (1826 - 1871) |
| Sibling | John Chambers (1828 - 1862) |
| Sibling | David Louis Chambers (1829 - 1858) |
| Sibling | Rachel M. Chambers (1830 - ) |
| Sibling | James K. Chambers (1830 - 1881) |
| Sibling | Joshua A. Chambers (1833 - 1910) |
| Sibling | Mary Jane Chambers (1835 - ) |