| Father | Willoughby Winchester II (1803 - 1873) |
| Mother | Mary Polly Prewitt (1811 - 1888) |
| Sibling | Mary Ann Winchester (1835 - 1920) |
| Sibling | Sam Winchester (1837 - 1862) |
| Sibling | John Thomas Winchester (1839 - 1862) |
| Sibling | Lydia Caroline Winchester (1841 - 1851) |
| Sibling | Edith Lodica Winchester (1843 - 1920) |
| Sibling | Malissa Ann Winchester (1845 - 1890) |
| Sibling | Richard Winchester (1846 - 1862) |
| Sibling | Emily Ann Winchester (1848 - 1922) |
| Sibling | Catherine Winchester (1850 - 1952) |
| Sibling | Martha Winchester (1852 - 1911) |
| Sibling | Thomas Prince Winchester (1852 - 1934) |
| Sibling | Elizabeth Jane Winchester (1854 - 1943) |
| Sibling | Margaret N. "Peggy" Winchester (1857 - 1905) |
| Sibling | James Nathaniel Winchester (1858 - 1864) |
| Sibling | Missouri Winchester (1859 - 1955) |
| Sibling | Alabama Clementine Winchester (1864 - 1951) |