| Father | John Knowlton Jr (1633 - 1684) |
| Mother | Deborah Grant (1633 - 1677) |
| Sibling | Joseph Knowlton (1651 - ) |
| Sibling | Samuel Knowlton (1653 - ) |
| Sibling | Deacon Nathaniel Knowlton (1658 - 1726) |
| Sibling | Elizabeth Knowlton (1659 - 1738) |
| Sibling | Thomas Knowlton (1662 - 1750) |
| Sibling | Abraham Knowlton (1664 - 1685) |
| Sibling | William Knowlton (1664 - ) |
| Sibling | Johnathan Knowlton III (1665 - 1720) |
| Sibling | Catherine Knowlton (1668 - 1768) |
| Sibling | Deborah Knowlton (1672 - 1750) |
| Sibling | Robert Knowlton (1672 - 1691) |
| Sibling | Susanna Knowlton (1673 - 1766) |
| Sibling | Ezekiel Knowlton (1679 - 1706) |