| Father | David R. Chambers Sr (1725 - 1808) |
| Mother | Margaret Ann Gaston (1725 - 1777) |
| Sibling | James Alexander Chambers (1748 - 1824) |
| Sibling | John C. Chambers Sr (1748 - 1830) |
| Sibling | Sgt William Joshua "Walkingstick Da-Sti-Sti" Chambers (1750 - 1840) |
| Sibling | Samuel Chambers (1752 - 1777) |
| Sibling | Joseph Gaston Chambers (1756 - 1829) |
| Sibling | Rev Alexander Chambers (1756 - 1857) |
| Sibling | David R. Chambers Jr (1757 - 1809) |
| Sibling | Jean Chambers (1759 - ) |
| Sibling | Rachel Chambers (1761 - 1843) |
| Sibling | Tetty Chambers (1765 - 1843) |