| Father | Jacob Reel Middlekauff (1789 - 1859) |
| Mother | Elizabeth A. Stonebraker (1795 - 1845) |
| Sibling | Lavinia Middlekauff (1813 - 1896) |
| Sibling | Susanna Middlekauff (1815 - 1816) |
| Sibling | Daniel Middlekauff (1817 - 1877) |
| Sibling | Esther Middlekauff (1819 - 1857) |
| Sibling | Samuel Stonebraker Middlekauff (1820 - 1872) |
| Sibling | Isabella Sarah Middlekauff (1826 - 1842) |
| Sibling | Mary Frances Middlekauff (1828 - 1891) |
| Sibling | Cecelia Barbara Middlekauff (1830 - 1842) |
| Sibling | Jacob Simon Middlekauff (1833 - 1863) |
| Sibling | Joseph Michael Middlekauff (1836 - 1909) |
| Sibling | Henry Fenton Middlekauff (1839 - 1862) |