| Father | Sir Christopher WILLOUGHBY (1453 - 1498) |
| Mother | Margaret JENNEY (1460 - 1515) |
| Sibling | Katherine WILLOUGHBY (1476 - 1542) |
| Sibling | Lady Elizabeth WILLOUGHBY (1473 - 1548) |
| Sibling | Sir Christopher WILLOUGHBY (1481 - 1539) |
| Sibling | Sir William WILLOUGHBY (1482 - 1526) |
| Sibling | George WILLOUGHBY (1484 - 1553) |
| Sibling | Sir Thomas Willoughby (1486 - 1545) |
| Sibling | Edmund WILLOUGHBY (1487 - ) |
| Sibling | Sir John WILLOUGHBY (1489 - ) |
| Sibling | Robert WILLOUGHBY (1490 - 1567) |
| Sibling | Richard WILLOUGHBY (1491 - 1546) |
| Sibling | Hugh WILLOUGHBY (1498 - ) |