| Father | Dom Manuel I Rei de Portugal (1469 - 1521) |
| Mother | Maria DE TRASTAMARA PRINSES VAN ARAGON (1482 - 1517) |
| Sibling | Infanta Maria Beatrice DO PORTUGAL Duchess Savoy (1504 - 1538) |
| Sibling | Dom João III , Rei de Portugal (1502 - 1557) |
| Sibling | Isabela DE PORTUGAL (1503 - 1539) |
| Sibling | Infante Louis DE AVIS E PORTUGAL DUQUE DE BEJA (1506 - 1555) |
| Sibling | Infante Fernando DE PORTUGAL (1507 - 1534) |
| Sibling | Cardenal Afonso DE AVIZ Infante of Portugal (1509 - 1540) |
| Sibling | Infanta María DE PORTUGAL (1511 - 1513) |
| Sibling | D. Henrique I DE PORTUGAL (1512 - 1580) |
| Sibling | Duque Duarte I DE PORTUGAL (1515 - 1540) |