| Father | Sir Thomas Howard 2nd Duke of Norfolk (1443 - 1524) |
| Mother | Lady Elizabeth Tilney Countess of Surrey & Norfolk (1445 - 1497) |
| Sibling | Thomas Howard 3rd Duke of Norfolk ( - ) |
| Sibling | Lady Elizabeth Howard Countess of Wiltshire, Ormond and Rochford ( - ) |
| Sibling | Henry Howard (1484 - 1513) |
| Sibling | Charles Howard (1483 - 1512) |
| Sibling | Muriel Howard (1486 - 1512) |
| Sibling | John Howard (1482 - 1503) |
| Sibling | Edmund Howard (1478 - 1539) |
| Sibling | Admiral Edward Howard (1476 - 1513) |
| Sibling | Richard Howard (1487 - 1517) |
| Sibling | Marcella Howard (1472 - 1497) |
| Sibling | Dorothy Howard (1465 - 1470) |