| Father | Sir Knight William Cecil 1st Lord Burghley, Lord High Treasurer & Chancellor of Cambridge University (1520 - 1598) |
| Mother | Baroness Mildred Cooke (1520 - 1589) |
| Sibling | Anne Cecil (1556 - 1588) |
| Sibling | Robert Cecil 1st Earl of Salisbury (1563 - 1612) |
| Sibling | Elizabeth Cecil (1559 - 1583) |
| Sibling | William Cecil (1559 - 1561) |
| Sibling | Francisca Cecil (1556 - 1558) |
| Sibling | Frances Cecil (1554 - 1554) |
| Sibling | Richard Cecil (1552 - 1553) |
| Sibling | Margaret Cecil (1562 - 1616) |
| Sibling | Catherine Cecil (1548 - 1567) |
| Sibling | Elizabeth Cecil (1564 - 1583) |
| Sibling | Thomas Cecil (1541 - ) |