| Spouse | Henry Valentine Hollingsworth (1598 - 1675) |
| Child | Robert Valentine Hollingsworth III (1632 - 1710) |
| Child | Henry Hollingsworth (1633 - 1713) |
| Child | Thomas Hollingsworth (1634 - 1701) |
| Child | Stephen Hollingsworth (1634 - 1734) |
| Child | Zebulon Hollingsworth (1636 - 1713) |
| Child | Ruth Hollingsworth (1638 - 1715) |
| Child | Mary Hollingsworth (1639 - 1654) |
| Child | Catherine Hollingsworth (1640 - 1646) |
| Child | Richard Hollingsworth (1640 - ) |
| Child | Abigail Hollingsworth (1642 - 1663) |
| Child | Mary Hollingsworth (1644 - ) |
| Child | Alice Hollingsworth (1649 - ) |