| Father | Josiah Levi Holden Gustin (1748 - 1810) |
| Mother | Margaret E. Wardner (1749 - 1857) |
| Sibling | Aaron Gustin (1790 - 1865) |
| Sibling | Caleb Gustin (1778 - 1778) |
| Sibling | Phillip Gustin (1781 - ) |
| Sibling | Aaron Gustin (1793 - 1891) |
| Sibling | Levi Holden Gustin (1798 - 1865) |
| Sibling | Josiah Gustin (1788 - 1865) |
| Sibling | Lydia Gustin (1783 - 1868) |
| Sibling | Margaret Gustin (1785 - 1809) |
| Sibling | Mary Polly Gustin (1790 - ) |
| Sibling | Sarah 'Sally' Gustin (1795 - ) |
| Sibling | Mary 'Molly' Gustin (1777 - 1863) |
| Sibling | Catherine Caroline Gustin (1779 - 1854) |