| Spouse | Anna Street (1710 - 1790) |
| Child | Peter Lester Jr. (1734 - 1789) |
| Child | Amos Lester (1734 - ) |
| Child | Anna Lester (1735 - 1807) |
| Child | Mary Lester (1736 - 1830) |
| Child | Anna Lester (1736 - ) |
| Child | Hannah Lester (1737 - 1800) |
| Child | Asa Lester (1738 - ) |
| Child | Amos Lester (1738 - ) |
| Child | John Lester (1740 - 1781) |
| Child | Nathan Lester (1742 - 1813) |
| Child | Lucy Lester (1744 - 1814) |
| Child | Elizabeth Lester (1746 - 1749) |
| Child | Eunice Leister (1748 - 1826) |
| Child | Deborah Lester (1750 - ) |
| Child | Sarah Lester (1753 - 1838) |
| Child | Mary Lester (1755 - ) |
| Child | Mollie Lester (1755 - ) |