| Father | William Doolittle (1795 - 1854) |
| Mother | Polly Ann Hubbell (1806 - 1886) |
| Sibling | Isaac Hubbell Doolittle (1824 - 1895) |
| Sibling | John Smith Doolittle (1824 - 1920) |
| Sibling | Frederick Benjamin Doolittle (1825 - 1912) |
| Sibling | Nathan Hezekiah Doolittle (1828 - 1829) |
| Sibling | James Augustus Doolittle (1831 - ) |
| Sibling | Nancy Araminta Doolittle (1832 - 1863) |
| Sibling | Sarah R. Doolittle (1833 - 1911) |
| Sibling | Monroe Doolittle (1836 - 1871) |
| Sibling | Mary Elizabeth Doolittle (1838 - 1839) |
| Sibling | Eunice Adelia Doolittle (1840 - 1851) |
| Sibling | Emeline Humeston Doolittle (1842 - 1903) |
| Sibling | James A. Doolittle (1827 - 1892) |