| Spouse | Philippa Grenville (1502 - 1571) |
| Child | Jane Tremayne (1540 - ) |
| Child | Richard Tremayne (1537 - 1584) |
| Child | Bridget Tremayne (1531 - ) |
| Child | Nicholas Tremayne (1535 - ) |
| Child | Catherine Tremayne (1527 - ) |
| Child | Digory Tremayne (1526 - 1601) |
| Child | Edmund Tremayne (1525 - 1582) |
| Child | Margaret Tremayne (1534 - ) |
| Child | Robert Tremayne (1529 - ) |
| Child | Mary Tremayne (1533 - ) |
| Child | Andrew Tremayne (1535 - ) |
| Child | John Tremayne (1537 - ) |
| Child | Mary Tremayne (1530 - 1583) |
| Child | Roger Tremayne (1524 - 1571) |
| Child | Bridget Tremayne (1524 - ) |
| Child | Richard Tremayne (1528 - 1584) |
| Child | John Tremayne (1529 - 1600) |
| Child | Jane Tremayne (1526 - ) |
| Child | Degori Tremayne (1525 - ) |
| Child | Robert Tremayne (1529 - ) |
| Child | Andrew Tremayne (1533 - 1564) |
| Child | Margaret Treymayne (1536 - ) |
| Child | Nicholas Tremayne (1533 - 1564) |
| Child | Catherine Tremayne (1517 - 1563) |