| Father | Elector Joachim I Nestor of Brandenburg (1484 - 1535) |
| Mother | Princess Elisabeth of Denmark, Sweden, and Norway (1485 - 1555) |
| Sibling | Johan Georg Von Brandenburg (1515 - 1597) |
| Sibling | Elector Joachim II Hector von Brandenburg (1505 - 1571) |
| Sibling | Princess Anna of Brandenburg, Duchess of Mecklenburg (1507 - 1567) |
| Sibling | Princess Elisabeth of Brandenburg, Duchess of Brunswick-Calenberg-Göttingen (1510 - 1558) |
| Sibling | Princess Margaret of Brandenburg, Duchess of Pomerania (1511 - 1577) |
| Sibling | Margrave John of Brandenburg-Küstrin (1513 - 1571) |