| Father | Sir Edmund Trafford (1485 - 1543) |
| Mother | Lady Elizabeth Longford (1487 - 1548) |
| Sibling | Elizabeth Trafford (1519 - 1582) |
| Sibling | Cecily de Trafford (1513 - 1572) |
| Sibling | Lady Margaret Gerard (1516 - 1540) |
| Sibling | Margaret Trafford (1516 - 1540) |
| Sibling | Rev Henry Trafford (1518 - 1591) |
| Sibling | Richard Trafford (1519 - 1569) |
| Sibling | George Trafford (1522 - 1572) |
| Sibling | Edmond IV Sir Trafford (1524 - 1563) |
| Sibling | Thomas Trafford (1528 - 1578) |
| Sibling | Elizabeth Trafford (1530 - 1556) |
| Sibling | Lady Alice Trafford (1537 - 1578) |