| Spouse | Eleanor Jephson (1520 - ) |
| Child | George Burley (1542 - ) |
| Child | Margaret Burley (1541 - ) |
| Child | Millicent Burley (1543 - ) |
| Child | Elizabeth Alice Burley (1544 - 1619) |
| Child | Christiania Burley (1544 - ) |
| Child | Richard Burley (1555 - ) |
| Child | Edward Burley (1557 - ) |
| Child | Robert Burley (1559 - ) |
| Child | Jane Burley (1561 - ) |
| Child | Joane Burley (1563 - ) |
| Child | Margaret Burley (1565 - 1632) |
| Child | Millycent Burley (1567 - ) |
| Child | Anne Burley (1568 - 1609) |
| Child | Dorothy Burley (1569 - 1650) |
| Child | Elizabeth Burley (1569 - ) |
| Child | Miss Burley (1571 - ) |