| Father | William Graham 3rd Earl of Menteith (1500 - 1544) |
| Mother | Margaret Mowbray of Barnbougle (1505 - 1548) |
| Sibling | Gilbert Graham of Beircrofts and Gartvertane (1510 - 1573) |
| Sibling | Allan Graham (1521 - 1573) |
| Sibling | Lady Margaret Mary Graham (1522 - 1556) |
| Sibling | Lawrence Graham (1522 - 1579) |
| Sibling | Andrew Graham (1522 - ) |
| Sibling | John Graham (1523 - 1565) |
| Sibling | Robert Graham Of Gartmore (1525 - 1573) |
| Sibling | Walter Graham (1527 - 1565) |
| Sibling | Christian Graham (1524 - 1597) |
| Sibling | David or George Grahame (1530 - ) |
| Sibling | Agnes Grahame (1531 - ) |
| Sibling | Marion Couttie Mentieth (1532 - 1587) |
| Sibling | Lady Catherine Kate Graham (1532 - 1630) |
| Sibling | William Graham (1536 - 1618) |