| Father | Gov. Nicholas Spencer III (1633 - 1689) |
| Mother | Frances Mottrom Bolton (1645 - 1720) |
| Sibling | William Spencer (1664 - 1705) |
| Sibling | John Spencer (1664 - ) |
| Sibling | Mottrom Spencer (1666 - 1703) |
| Sibling | Nicholas Spencer Jr. (1667 - ) |
| Sibling | Letticia Spencer (1667 - 1702) |
| Sibling | William Spencer (1668 - 1699) |
| Sibling | Frances Spencer (1670 - ) |
| Sibling | John Spencer (1672 - 1708) |
| Sibling | Nicholas Spencer Jr. (1672 - 1723) |
| Sibling | Elizabeth Spencer (1672 - 1730) |
| Sibling | Francis Spencer (1674 - 1720) |
| Sibling | Alexander Spencer (1676 - ) |
| Sibling | Elizabeth Emilie Spencer (1677 - 1780) |
| Sibling | Mary Frances Spencer (1683 - 1721) |
| Sibling | Nicholas Spencer (1685 - 1777) |
| Sibling | William Nicholas Spencer Jr (1685 - 1740) |
| Sibling | John Spencer (1685 - 1777) |
| Sibling | John Spencer (1687 - 1725) |
| Sibling | Francis Spencer (1687 - 1720) |
| Sibling | Alexander Spencer (1689 - ) |
| Sibling | Elizabeth Ann Spencer (1690 - 1764) |
| Sibling | William Spencer ( - 1695) |
| Sibling | John Spencer ( - ) |