| Father | High Sheriff Robert Throckmorton (1510 - 1581) |
| Mother | Elizabeth Hussey (1528 - 1572) |
| Sibling | Muriel Throckmorton (1547 - 1615) |
| Sibling | Mary Throckmorton (1569 - 1573) |
| Sibling | Robert Throckmorton Jr. (1556 - ) |
| Sibling | Anne Throckmorton ( - 1605) |
| Sibling | Elizabeth Throckmorton (1554 - ) |
| Sibling | George Throckmorton (1552 - ) |
| Sibling | Temperance Frances Throckmorton (1553 - 1611) |
| Sibling | Alice Throckmorton (1544 - 1610) |
| Sibling | Roger Throckmorton (1545 - ) |
| Sibling | Margaret Throckmorton (1568 - 1610) |
| Sibling | Margaret Throckmorton (1560 - ) |