| Spouse | Lady Anne Greenway (1515 - 1586) |
| Child | Lord John Howland III (1541 - 1611) |
| Child | Robert Howland (1545 - 1621) |
| Child | Humphrey Howland (1549 - 1646) |
| Child | Sir Giles Howland (1549 - 1612) |
| Child | Henry Howland (1564 - ) |
| Child | Michael Howland (1553 - 1632) |
| Child | Nicholas Holland (1555 - 1611) |
| Child | Benjamin Howland (1550 - 1558) |
| Child | Richard Howland (1540 - 1600) |
| Child | Geoffrey Howland (1547 - ) |
| Child | Christian Howland (1544 - 1608) |
| Child | George Howland (1543 - 1653) |
| Child | Ralf Howland (1546 - 1608) |
| Child | William Howland (1554 - 1601) |
| Child | James Howland (1561 - ) |
| Child | Benjamin Howland (1542 - 1548) |
| Child | William Howland (1542 - 1587) |
| Child | Christiana Howland (1544 - 1587) |
| Child | Raffe Howland (1548 - 1587) |