| Father | Lord James Stirling 4th of Keir and 1st of Cadder (1517 - 1588) |
| Mother | Baroness Jean Chisholm (1517 - 1603) |
| Sibling | William Stirling (1533 - ) |
| Sibling | Helen Stirling (1540 - 1605) |
| Sibling | Margaret Stirling or Striveling (1540 - ) |
| Sibling | John Stirling (1541 - ) |
| Sibling | James Stirling (1543 - ) |
| Sibling | Sir Archibald Stirling (1546 - 1630) |
| Sibling | Barbara Stirling (1548 - ) |
| Sibling | William Cornelius Stirling (1549 - 1576) |
| Sibling | Lady Elizabeth Sterling (1554 - 1579) |
| Sibling | Agnes Chisholm (1567 - 1617) |
| Sibling | Mary Cochan (1569 - 1629) |