| Father | Baron Robert Edmund Spencer Baron of Cople (1533 - 1620) |
| Mother | Rose Cockayne (1556 - 1629) |
| Sibling | Jane Spencer (1572 - ) |
| Sibling | Nicholas I, of Cople Spencer (1573 - 1626) |
| Sibling | John Spencer (1574 - 1626) |
| Sibling | William Spencer (1576 - 1638) |
| Sibling | George Spencer (1579 - 1630) |
| Sibling | Margaret Spencer (1579 - ) |
| Sibling | Robert Spencer (1580 - 1594) |
| Sibling | Ann Spencer (1585 - 1594) |
| Sibling | Elizabeth Spencer (1585 - ) |
| Sibling | Arnold Spencer (1587 - 1658) |
| Sibling | Edward Spencer (1590 - ) |
| Sibling | Cicely Spencer (1592 - 1656) |
| Sibling | Rose Spencer (1594 - ) |