| Father | John Ayer (1582 - 1657) |
| Mother | Hannah Everd (1582 - 1688) |
| Sibling | Mary Ayres (1607 - 1702) |
| Sibling | John Jr Ayers (1616 - 1675) |
| Sibling | Sarah Ayers (1621 - 1692) |
| Sibling | Robert Ayer (1625 - 1711) |
| Sibling | Thomas Ayer (1626 - 1686) |
| Sibling | Rebecca Ayer (1627 - 1718) |
| Sibling | John Ayer (1633 - 1675) |
| Sibling | Mary Agnes Ayer (1634 - 1670) |
| Sibling | Obediah Ayer (1635 - 1694) |
| Sibling | Obediah Ayers (1635 - 1694) |
| Sibling | Susannah Ayers (1636 - 1676) |
| Sibling | Alexander Ayer (1639 - 1723) |
| Sibling | Nathaniel Ayers (1644 - 1717) |
| Sibling | Rebecca Ayer (1646 - ) |
| Sibling | Thomas Ayer (1650 - 1671) |
| Sibling | Nathaniel Ayer (1655 - 1717) |