| Father | Asa Chamberlain (1793 - 1858) |
| Mother | Sally Pratt Russell (1796 - 1860) |
| Sibling | Rhoda Salinda Chamberlain (1819 - 1850) |
| Sibling | Jedediah Russell Chamberlain (1820 - 1896) |
| Sibling | Sarah Minerva Chamberlain (1821 - 1897) |
| Sibling | Asa Riley Chamberlain (1823 - 1896) |
| Sibling | William Russell Chamberlain (1827 - 1880) |
| Sibling | Eliza Jane Chamberlain (1829 - 1907) |
| Sibling | Ann Chloe Chamberlain (1832 - 1895) |
| Sibling | Catherine J. "Kate" Chamberlain (1835 - 1868) |
| Sibling | Augusta Celina Chamberlain (1838 - 1918) |
| Sibling | Austin Eliab Chamberlain (1841 - 1914) |