| Father | Abiel Chamberlain (1737 - 1818) |
| Mother | Grace Ainsworth (1743 - 1788) |
| Sibling | Sarah Chamberlain (1762 - 1827) |
| Sibling | Marsilva Chamberlain (1764 - 1822) |
| Sibling | Mary "Polly" Chamberlain (1766 - ) |
| Sibling | Zerviah Chamberlain (1768 - 1843) |
| Sibling | Joanna Chamberlain (1770 - 1846) |
| Sibling | Willoba Chamberlain (1772 - 1852) |
| Sibling | Abiel Chamberlain (1774 - 1846) |
| Sibling | Eunice Chamberlain (1775 - 1849) |
| Sibling | Olive Chamberlain (1777 - 1868) |
| Sibling | Huldah Peake Chamberlain (1779 - 1840) |
| Sibling | William Chamberlain (1782 - 1853) |
| Sibling | Rensselaer Chamberlain (1788 - ) |
