| Father | Sir John SEYMOUR of Wulfhall,Courtier to King Henry VIII, Father of Jane (1474 - 1536) |
| Mother | Margery WENTWORTH (1478 - 1550) |
| Sibling | Jane SEYMOUR Queen of England (1508 - 1537) |
| Sibling | Dorothy SEYMOUR (1513 - 1566) |
| Sibling | Sir Thomas SEYMOUR 1st Baron Seymour of Sudeley (1512 - 1549) |
| Sibling | Elizabeth SEYMOUR Marchioness of Winchester, Lady Cromwell (1520 - 1563) |
| Sibling | Sir Henry SEYMOUR (1507 - 1578) |
| Sibling | Sir John SEYMOUR V. (1500 - 1510) |
| Sibling | Margery SEYMOUR (1502 - 1520) |
| Sibling | Sir Edward SEYMOUR (1506 - 1552) |